WHAT WE CAN DOIn vitro Efficacy testing
We offer standard and custom-made in vitro and ex vivo protocols to provide the most reliable and cost-effective testing services for finished products and active ingredients
FAST RESPONSE AND IMMEDIATE ATTENTIONAntioxidant Capacity
The antioxidant potential (AOP) of the skin is known to be a decisive factor in skin aging. External aggressors such as stress or sun irradiation induce radicals and biochemical changes, thus affecting the AOP of cells and skin.
Dermaclaim has different in vitro models at your disposal:
- Immortalized human keratinocytes (HaCaT)
- Normal Human Epidermal Keratinocytes (NHEK)
- Normal Human Dermal Fibroblasts (NHDF)
- Reconstructed Human Epidermis (RHE, RHE FT)
- In tubo colorimetric platforms
We can evaluate the following biomarkers:
- mRNA gene expression (SOD1, CAT, GPx1, NRF2…)
- Glutathione levels (GSH-GSSG)
- Reactive Oxygen/Nytrogen Species (ROS and RNS)
- ORAC, TEAC
ONE OF THE BEST SCIENTIFIC REPORTS IN THE MARKETSkin Ageing and Protection
Ageing is a complex and multi-factorial process that leads to deep changes in the skin structure and function. In Dermaclaim, we explore new dermocosmetics strategies which might prevent or decelerate the signs of ageing.
Dermaclaim has different in vitro models at your disposal:
- Immortalized human keratinocytes (HaCaT)
- Normal Human Epidermal Keratinocytes (NHEK)
- Normal Human Dermal Fibroblasts (NHDF)
- Reconstructed Human Epidermis (RHE, RHE FT)
- In tubo colorimetric platforms
We can evaluate the following biomarkers:
- Extracellular matrix proteins (collagen, elastin, HA, MMPs, versican…)
- Oxidative stress and DNA damage (ROS, MTT, thymine dimers, Y-H2AX)
- Senescence markers (telomeres, TERT, TEP1, B-gal, SIRT1)
- Oxidative damages (protein carbonylation, lipid peroxidation, protein glycation)
- Cell cycle & autophagy markers (CLOCK, BMAL, PER, LMNB1, CDKN1A, LC3…)
- Proteasome activity (clusterin, APP, chymotrypsin-like)
LATEST EQUIPMENTS AND CUTTING-EDGE TECHNOLOGIESHydration, Epidermal Barrier and Wound-healing
Skin protects us from external environmental aggressions. Through its barrier function, skin is our shield and prevents the evaporation of essential elements such as water. Skin hydration is mostly linked to the skin barrier function.
Dermaclaim has different in vitro models at your disposal:
- Immortalized human keratinocytes (HaCaT)
- Normal Human Epidermal Keratinocytes (NHEK)
- Normal Human Dermal Fibroblasts (NHDF)
- Reconstructed Human Epidermis (RHE, RHE FT)
We can evaluate the following biomarkers:
- Cell cytotoxicity and proliferation (MTT, BrdU, NRU)
- Epidermal differentiation biomarkers (filaggrin, involucrin, cytokeratins…)
- Barrier function biomarkers (hyaluronic acid, ceramides, aquaporines, GAGs…)
- Wound-healing (Scratch-assay)
- Epidermal cohesion biomarkers (integrin, occludin, claudin, collagens, connexins…)
HIGH QUALITY-TO-COST RATIOHair growth, alopecia & gray
Hair fall is a widespread problem among all genders, ages, and ethnicity with both physical and psychological effects. Different parameters related to hair loss, hair growth and hay graying can be analyzed using different technologies.
Dermaclaim has different in vitro models at your disposal:
- Human Follicle Dermal Papilla Cells (HFDPC)
- Human Hair Follicle Keratinocytes (HHFK)
- Human follicles | Human hair swatches (ex vivo)
We can evaluate the following biomarkers:
- Cell proliferation and growth (MTT)
- 5alpha-Reductase (SRD5A1, SRD5A2, SRD5A3)
- Growth factors (EDN1, HDC, FGF1, VEGF, KGF, IGF1…)
- Hair keratins (KRT31, KRT40, KRT85, KRT86…)
- Specific marker expression (Ki67, B-catenin, apoptosis, KRTs…)
- Hair graying biomarkers (IRF4, MITF, TYR, TYRP-1)
FULL TRANSPARENCY (COMPLETE ACCESS TO RAW DATA)Skin pigmentation
Melanogenesis (synthesis of melanin) is the primary cause of human skin and hair pigmentation. Whitening and pro-pigmenting agents are widely used in cosmetics to obtain lighter/tanner complexion or treatment of skin disorders.
Dermaclaim has different in vitro models at your disposal:
- Normal Human Epidermal Melanocytes (NHEM)
- Melanocyte cell lines (B16)
- Reconstructed Human Pigmented Epidermis (RHPE)
- Co-culture of keratinocytes and melanocytes (NHEK/NHEM)
- In tubo colorimetric platforms
We can evaluate the following biomarkers:
- Melanin content
- Melanogenesis pathways (TYR, TYRP-1, TYRP-2, MITF, POMC, Pmel17…)
- Tyrosinase enzymatic activity (in tubo)
- Fontana-Masson staining and image analysis quantification
SCIENTIFIC RELIABILITY FOCUSED ON YOUR MARKETINGAnti-acne, Oily skin and Slimming
Oily skin results from an excessive sebum synthesis in the sebocytes of the sebaceous glands. An overproduction of sebum is one of the most common dermatologic concerns and may lead to acne prone skin.
Dermaclaim has different in vitro models at your disposal:
- iPSC-derivates sebocytes (induced Pluripotent Stem Cell)
- Human Adipocytes (HPAd, HAd)
- Mouse Adipocytes (3T3-L1)
- Normal Human Dermal Fibroblasts (NHDF)
We can evaluate the following biomarkers:
- Total fatty acid content (Oil Red O staining)
- Lipogenesis mRNA gene expression (ATGL, HSL, PRDM, UCP…)
- Sebocyte differentiation and maturation (EMA, KRT7…)
- 5-alpha Reductase (SRD5A1-3) activity and testosterone metabolism
- Inflammatory response after induction with P.acnes
- Cholesterol and adiponectin levels
IMPROVED TECHNICAL ASSAY CONDITIONSSkin inflammation
Skin inflammation is a sign of an immune response in the body. The cause or trigger of skin inflammation may be acute, such as a skin infection, or chronic, such as an autoimmune condition like psoriasis.
Dermaclaim has different in vitro models at your disposal:
- Immortalized human keratinocytes (HaCaT)
- Normal Human Epidermal Keratinocytes (NHEK)
- Normal Human Dermal Fibroblasts (NHDF)
- Reconstructed Human Epidermis (RHE, RHE FT)
- Peripheral Blood Mononuclear Cells (PBMC)
- CD4+ lymphocytes / CD8+ T-cells
- Human Dendritic Cells (HDC)
We can evaluate the following biomarkers, using or not external aggressors (LPS, histamine, UV, PMA):
- Inflammation biomarkers (TNFa, IL1a, IL1b, IL6, IL8, IL10, ICAM, TGFb…)
- Transcription factors (NFkB, AP1, STAT1, COX2, ALOX5…)
- Pain inhibition and singalling (TRPV1, TNFa)
KNOW-HOW WITH +10 YEARS OF EXPERTISEAnti-Pollution
Exposure of the skin to air pollutants (PM, PAHs, VOCs, O3, etc.) has been associated with skin aging and inflammatory or allergic skin conditions such as atopic dermatitis, eczema, psoriasis or acne.
Dermaclaim has different in vitro models at your disposal:
- Immortalized human keratinocytes (HaCaT)
- Normal Human Epidermal Keratinocytes (NHEK)
- Normal Human Dermal Fibroblasts (NHDF)
- Reconstructed Human Epidermis (RHE, RHE FT)
- Human Bronchial Fibroblasts Cells (HBFC)
We can evaluate the following biomarkers, using or not external aggressors (PM, heavy metals, PHA):
- Cell viability (MTT) after urban dust exposure
- Oxidative stress (ROS)
- Inflammatory mediators (TNFa, IL1a, IL6, IL8, PGE2…)
- Apoptosis-induced biomarkers (CASP9, BAX2, ARNTL…)
- Xenobiotic markers (AhR-CYP1A1, NRF2)
FLEXIBILITY AND CUSTOM-MADE BIOASSAYSDermal Penetration
Dermal penetration bioassays are essential to provide evidence of how effectively active ingredients or end-products penetrate the skin to deliver their intended benefits, ensuring product efficacy and safety.
Dermaclaim has different in vitro models at your disposal:
- Human cell lines (HaCaT, NHEK, NHDF, HFDPC…)
- 3D Reconstructed Human Epidermis (RHE)
- 3D Reconstructed Full-Thickness Skin (RHE FT)
- Adult Human Skin Biospsies 12mm (HSE)
We can evaluate dermal penetration using immunohistochemistry after including fluorophores (fluorescein, rhodamine..) in the tested formulation, using different stainings
- DAPI (cell nuclei)
- Phalloidin (cytoplasm)
- Green / Red Fluorescence (test sample)
SCREENING FOR ACTIVE INGREDIENTSTranscriptomics
Analysis of the effects on the expression of whole human transcriptome (3'mRNA sequencing) or targeted-qPCR, especially useful for screening and determination of the potential effects of new active ingredients.
Dermaclaim has different in vitro models at your disposal:
- Immortalized human keratinocytes (HaCaT)
- Normal Human Epidermal Keratinocytes (NHEK)
- Normal Human Dermal Fibroblasts (NHDF)
- Reconstructed Human Epidermis (RHE, RHE FT)
We can evaluate the whole human transcriptomics or targeted-qPCR for specific panels of genes:
- 3’mRNA sequencing (>20.000 genes | > 100.000 different transcripts).
- Targeted qPCR (pre-established or customized panels of 10, 20, 50, 100 genes).
BASIC RESEARCH APPLICATIONSCollagen Ecosystem
Healthy skin depends on the amount, organization and functional integration of multiple extracellular matrix components. A multi-collagen profile therefore provides a broader mechanistic view than the assessment of COL1A1 alone.
Type I and III collagens form the principal dermal fibrillar scaffold, while additional collagen families regulate fibrillogenesis, tissue architecture, mechanical properties and dermal–epidermal cohesion. A multi-collagen profile therefore provides a broader mechanistic view than the assessment of COL1A1 alone, what could be assessed on:
- Normal Human Dermal Fibroblasts (NHDF)
- 3D Reconstructed Full-Thickness Skin (RHE FT)
We can evaluate the stimulating capacity on collagen ecosystem (10 different collagens), through gene expression assessment using qPCR or protein synthesis quantification using ELISA:
- Gene expression: 10 genes (qPCR)
- Protein levels: 10 proteins (ELISA)
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